Product Name :
ApoF (E258) polyclonal antibody Background :
Apolipoproteins are a family of fatty-acid binding proteins that transport fat through the bloodstream in the form of lipoproteins. ApoF (apolipoprotein F), also known as LTIP (lipid transfer inhibitor protein), is a 308 amino acid secreted protein that belongs to the apolipoprotein family. Expressed in liver tissues and also existing in plasma, ApoF inhibits the activity of CETP (cholesteryl ester transfer protein) and, via this inhibition, functions to regulate cholesterol transport. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ApoF (E258) polyclonal antibody detects endogenous levels of ApoF protein. Immunogen :
Synthetic peptide, corresponding to amino acids 226-279 of Human ApoF. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of ApoF (E258) pAb at 1:1000 dilution Lane1:HepG2 whole cell lysate(10ug) Lane2:L02 whole cell lysate(10ug) Lane3:AML-12 whole cell lysate(40ug) Lane4:H9C2 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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