Product Name :
ACAT1 (K266) polyclonal antibody Background :
ACAT-1 (acetyl-Coenzyme A acetyltransferase 1) is a mitochondrial enzyme involved in the formation and degradation of ketone bodies and is necessary for the proper metabolic processing of isoleucine. Rare defects in the gene encoding ACAT-1 lead to β-ketothiolase deficiency, which is characterized by ketoacidotic attacks. ACAT-2 (acetyl-Coenzyme A acetyltransferase 2) is considered a cytosolic protein and is crucial for cholesterol synthesis. Specifically, both Acetoacetyl-CoA specific thiolases, ACAT-1 and ACAT-2 catalyze the formation of acetoacetyl-CoA from two acetyl-CoA molecules. These enzymes are also capable of the reverse reaction, the cleavage of acetoacetyl-CoA into two acetyl-CoA molecules. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ACAT1 (K266) polyclonal antibody detects endogenous levels of ACAT1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 235-286 of Human ACAT1. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of ACAT-1 (K266) pAb at 1:1000 dilution Lane1:HEK293T whole cell lysate(20ug) Lane2:HepG2 whole cell lysate(20ug) Lane3:The Lung tissue lysate of Rat(20ug) Lane4:The Lung tissue lysate of Mouse(10ug)
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  Immunohistochemistry (IHC) analyzes of ACAT1 (K266) pA bin paraffin-embedded human liver carcinoma tissue at 1:50,showing cytoplasmic staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
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  Immunohistochemistry (IHC) analyzes of ACAT1 (K266) pA bin paraffin-embedded human liver carcinoma tissue at 1:50,showing cytoplasmic staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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