Product Name :
PSMD12 (I186) polyclonal antibody Background :
PSMD12 (proteasome (prosome, macropain) 26S subunit, non-ATPase, 12), also known as p55 or Rpn5, is a 456 amino acid protein belonging to the proteasome subunit p55 family. PSMD12 acts as a regulatory subunit of the 26S Proteasome and is a component of the PA700 complex. PSMD12 contains one PCI domain. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PSMD12 (I186) polyclonal antibody detects endogenous levels of PSMD12 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 161-210 of Human PSMD12. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of PSMD12 (I186) polyclonal antibody at 1:1000 dilution Lane1:HELA whole cell lysate(42ug) Lane2:LO2 whole cell lysate(38ug) Lane3:The Kidney tissue lysate of Rat(47ug) Lane4:The Kidney tissue lysate of Mouse(37ug)
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  Immunohistochemistry (IHC) analyzes of PSMD12 (I186) pAb in paraffin-embedded human breast cancer tissue.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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