p27 Kip1 (A181) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
p27 Kip1 (A181) polyclonal antibody Background :
Cell cycle progression is regulated by a series of cyclin-dependent kinases that consist of catalytic subunits, designated Cdks, and activating subunits, designated cyclins. Orderly progression through the cell cycle requires the activation and inactivation of different cyclin-Cdks at appropriate times. A series of proteins has been recently described that function as “mitotic inhibitors.” These include p21, the levels of which are elevated upon DNA damage in G1 in a p53-dependent manner, p16 and a more recently described p16 related inhibitor designated p15. A p21 related protein, p27, has been described as a negative regulator of G1 progression and has been speculated to function as a possible mediator of TGFβ-induced G1 arrest. p27 interacts strongly with D-type cyclins and Cdk4 in vitro and to a lesser extent with cyclin E and Cdk2. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p27 Kip1 (A181) polyclonal antibody detects endogenous levels of p27 Kip1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 150-200 of Human p27 Kip1. Conjugate :
Unconjugated Modification :
Unmodification
p27 Kip1 (A181) polyclonal antibody Background :
Cell cycle progression is regulated by a series of cyclin-dependent kinases that consist of catalytic subunits, designated Cdks, and activating subunits, designated cyclins. Orderly progression through the cell cycle requires the activation and inactivation of different cyclin-Cdks at appropriate times. A series of proteins has been recently described that function as “mitotic inhibitors.” These include p21, the levels of which are elevated upon DNA damage in G1 in a p53-dependent manner, p16 and a more recently described p16 related inhibitor designated p15. A p21 related protein, p27, has been described as a negative regulator of G1 progression and has been speculated to function as a possible mediator of TGFβ-induced G1 arrest. p27 interacts strongly with D-type cyclins and Cdk4 in vitro and to a lesser extent with cyclin E and Cdk2. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p27 Kip1 (A181) polyclonal antibody detects endogenous levels of p27 Kip1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 150-200 of Human p27 Kip1. Conjugate :
Unconjugated Modification :
Unmodification
-
-
Immunohistochemistry (IHC) analyzes of p27 Kip1 (A181) pAb in paraffin-embedded human brain tissue.
HDAC1 and Klf4 interplay critically regulates human myeloid leukemia cell proliferation
PMCID: Pubmed No.:25341045
HOXB7 as a prognostic factor and mediator of colorectal cancer progression
PMCID: Pubmed No.:21474578
MCM-2 is a therapeutic target of Trichostatin A in colon cancer cells
PMCID: Pubmed No.:23770000
MicroRNA-302b suppresses cell proliferation by targeting EGFR in human hepatocellular carcinoma SMMC-7721 cells.
PMCID: Pubmed No.:24083596
MicroRNA-30b functions as a tumour suppressor in human colorectal cancer by targeting KRAS, PIK3CD and BCL2
PMCID: Pubmed No.:24293274
MIF4G domain containing protein regulates cell cycle and hepatic carcinogenesis by antagonizing CDK2-dependent p27 stability
PMCID: Pubmed No.:24336329
MiR-222 overexpression promotes proliferation of human hepatocellular carcinoma HepG2 cells by downregulating p27
PMCID: Pubmed No.:24955159
Protein Tyrosine Phosphatase U (PTPRU) is Required for Glioma Growth and Motility
PMCID: Pubmed No.:24876153
MicroRNA-221 is Required for Proliferation of Mouse Embryonic Stem Cells via P57 Targeting
PMCID: Pubmed No.:25086570
HDAC1 and Klf4 interplay critically regulates human myeloid leukemia cell proliferation
PMCID: Pubmed No.:25341045
Let-7f Inhibits Glioma Cell Proliferation, Migration, and Invasion by Targeting Periostin.
PMCID: Pubmed No.:25735962
MicroRNA-222 promotes human non-small cell lung cancer H460 growth by targeting p27
PMCID: Pubmed No.:26131134
MicroRNA-222 Controls Human Pancreatic Cancer Cell Line Capan-2 Proliferation by P57 Targeting
PMCID: Pubmed No.:26535064
MicroRNA-221 is Required for Proliferation of Mouse Embryonic Stem Cells via P57 Targeting
PMCID: Pubmed No.:25086570
HDAC1 and Klf4 interplay critically regulates human myeloid leukemia cell proliferation
PMCID: Pubmed No.:25341045
MicroRNA-302b suppresses cell proliferation by targeting EGFR in human hepatocellular carcinoma SMMC-7721 cells.
PMCID: Pubmed No.:24083596
MicroRNA-222 Controls Human Pancreatic Cancer Cell Line Capan-2 Proliferation by P57 Targeting
PMCID: Pubmed No.:26535064
MicroRNA-30b functions as a tumour suppressor in human colorectal cancer by targeting KRAS, PIK3CD and BCL2
PMCID: Pubmed No.:24293274
MIF4G domain containing protein regulates cell cycle and hepatic carcinogenesis by antagonizing CDK2-dependent p27 stability
PMCID: Pubmed No.:24336329
KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells
PMCID: Pubmed No.:29520102
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.