Product Name :
SEMA3C (E750) polyclonal antibody Background :
SEMA3C (also designated Semaphorin 3C, Semaphorin E, SEMAE, SemE,sema domain, immunoglobulin domain (Ig) and short basic domain, secreted)is an ubiquitous protein of approximately 70 kDa that mediates axonal guidance, repulsive gradients, induction of growth cone collapse and cell survival/death. Secreted SEMA3C favors survival and neuritogenesis of cultured cerebellar granule neurons (CGNs). SEMA3C from macrophages and fibroblasts that selectively directs against sympathetic nerve fibers may be one element responsible for reduced sympathetic innervation in rheumatoid arthritis tissue.SEMA3C mutant mice die within hours after birth from congenital cardiovascular defects consisting of interruption of the aortic arch and improper septation of the cardiac outflow tract. SEMA3C is expressed in the cardiac outflow ract as neural crest cells and promotes crest cell migration into the proximal cardiac outflow tract. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
SEMA3C (E750) polyclonal antibody detects endogenous levels of SEMA3C protein. Immunogen :
Synthetic peptide, corresponding to amino acids 713-768 of Human SEMA3C. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of SEMA3C (E750) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate
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  Immunohistochemistry (IHC) analyzes of SEMA3C (E750) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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