Product Name :
IL-32 (K226) polyclonal antibody Background :
Interleukin-32 (IL-32), also designated Natural Killer (NK) cell transcript 4 (nk4), may play a role in inflammatory responses such as lymphocyte activation. IL-32 is secreted following stimulation with inflammatory cytokines such as IL-1β and IFN-γ, and by NK cells after exposure to IL-12 and IL-18. It also induces production of IL-1β, TNFα, MIP-2 and IL-6. Expression of IL-32 increases after activation of T cells by mitogens or activation of NK cells by IL-2. The IL-32 protein contains three potential N-myristoylation sites, a tyrosine sulfation site, an RGD cellattachment sequence and multiple putative phosphorylation sites. Human IL-32 exists as four isoforms. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
IL-32 (K226) polyclonal antibody detects endogenous levels of IL-32 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 200-2450 of Human IL-32. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of IL-32 (K226) pAb at 1:500 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:LO2 whole cell lysate(20ug) Lane3:SGC7901 whole cell lysate(40ug) Lane4:PMVEC whole cell lysate(40ug) Lane5:AML-12 whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of IL-32 (K226) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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