AQP3 (V214) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
AQP3 (V214) polyclonal antibody Background :
AQP3 is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
AQP3 (V214) polyclonal antibody detects endogenous levels of AQP3 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 180-228 of Human AQP3. Conjugate :
Unconjugated Modification :
Unmodification
AQP3 (V214) polyclonal antibody Background :
AQP3 is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
AQP3 (V214) polyclonal antibody detects endogenous levels of AQP3 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 180-228 of Human AQP3. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of AQP3 (V214) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:A375 whole cell lysate(40ug) Lane3:The Kidney tissue lysate of Mouse(40ug) Lane4:The Kidney tissue lysate of Rat(40ug) -
Immunohistochemistry (IHC) analyzes of AQP3 (V214) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Immunohistochemical distribution and morphometric analysis of aquaporin-3 in oral squamous cell carcinoma
PMCID: Pubmed No.:23830572
Forensic diagnosis of ante-and postmortem burn based on aquaporin-3 gene expression in the skin
PMCID: Pubmed No.:24508472
Calcium signaling and cell volume regulation are altered in Sjögren's Syndrome
PMCID: Pubmed No.:24471729
Forensic diagnosis of ante-and postmortem burn based on aquaporin-3 gene expression in the skin
PMCID: Pubmed No.:24508472
Calcium signaling and cell volume regulation are altered in Sjögren's Syndrome
PMCID: Pubmed No.:24471729
AQP3 small interfering RNA and PLD2 small interfering RNA inhibit the proliferation and promote the apoptosis of squamous cell carcinoma
PMCID: Pubmed No.:28656282
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.