Product Name :
ERK1/2 (H197) polyclonal antibody Background :
The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and Threonine sites mapping within a characteristic Thr- Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phos-phorylate downstream components on Serine and Threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ERK1/2 (H197) polyclonal antibody detects endogenous levels of ERK1/2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 165-210 of Human ERK1. Conjugate :
Unconjugated Modification :
Unmodification
ERK1/2 (H197) polyclonal antibody Background :
The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and Threonine sites mapping within a characteristic Thr- Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phos-phorylate downstream components on Serine and Threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ERK1/2 (H197) polyclonal antibody detects endogenous levels of ERK1/2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 165-210 of Human ERK1. Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot (WB) analysis of ERK1/2 (H197) at 1:2000 dillution Lane1:Hela whole cell lysate(15μg) Lane2:NIH-3T3 whole cell lysate(40μg) Lane3:H9C2 whole cell lysate(15μg)
-
Leptin relieves intestinal ischemia/reperfusion injury by promoting ERK1/2 phosphorylation and the NO signaling pathway.
PMCID: Pubmed No.:21857261
Hypoxia-induced apoptosis is blocked by adrenomedullin via upregulation of Bcl-2 in human osteosarcoma cells
PMCID: Pubmed No.:26035796
Effects of Ixeris dentata water extract and caffeic acid on allergic inflammation in vivo and in vitro
PMCID: Pubmed No.:26104582
Ruscogenin suppresses mouse neutrophil activation: Involvement of protein kinase A pathway
PMCID: Pubmed No.:26134424
Protective effect of butyrate against ethanol-induced gastric ulcers in mice by promoting the anti-inflammatory, anti-oxidant and mucosal defense mechanisms
PMCID: Pubmed No.:26604089
Protective effect of butyrate against ethanol-induced gastric ulcers in mice by promoting the anti-inflammatory, anti-oxidant and mucosal defense mechanisms
PMCID: Pubmed No.:26604089
Astragaloside IV prevents kidney injury caused by iatrogenic hyperinsulinemia in a streptozotocin‑induced diabetic rat model
PMCID: Pubmed No.:29207011
Signal transduction involved in lipoxin A4‑induced protection of tubular epithelial cells against hypoxia/reoxygenation injury
PMCID: Pubmed No.:28259922
Inhibition of MAD2B alleviates venous neointimal formation by suppressing VSMCs proliferation and migration
PMCID: Pubmed No.:34605572
A phycocyanin derived eicosapeptide attenuates lung fibrosis development
PMCID: Pubmed No.:34280398
CXCR3 expression in colorectal cancer cells enhanced invasion through preventing CXCR4 internalization
PMCID: Pubmed No.:30092218
CGFe and TGF‑β1 enhance viability and osteogenic differentiation of human dental pulp stem cells through the MAPK pathway
PMCID: Pubmed No.:34434262
Muscone and (+)-Borneol Cooperatively Strengthen CREB Induction of Claudin 5 in IL-1β-Induced Endothelium Injury
PMCID: Pubmed No.:35892657
Puerarin alleviates depressive-like behaviors in high-fat diet-induced diabetic mice via modulating hippocampal GLP-1R/BDNF/TrkB signaling
PMCID: Pubmed No.:36039913
Anti-oxidant and Anti-inflammatory Effects of Ethanol Extract from Polygala sibirica L. var megalopha Fr. on Lipopolysaccharide-Stimulated RAW264.7 Cells
PMCID: Pubmed No.:37434032
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.