Op18 (A18) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
Op18 (A18) polyclonal antibody Background :
Op18 (for oncoprotein 18, also designated stathmin, prosolin or metablastin) is a conserved, tubulin-associated, intracellular phosphoprotein. Many different phosphorylated forms of Op18 are observed, and it is expressed as two different isoforms. Op18 is considered a critical regulator of microtubulin dynamics and is downregulated by p53. It serves as a transducing protein, via phosphorylation, for a variety of cell signaling pathways and is involved in both mitosis and differentiation. Op18 is present in many cancers, including breast carcinomas, and is highly expressed in acute leukemias of different subtypes. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Op18 (A18) polyclonal antibody detects endogenous levels of Op18 protein. Immunogen :
Synthetic peptide, corresponding to the N-terminus of Human Op18. Conjugate :
Unconjugated Modification :
Unmodification
Op18 (A18) polyclonal antibody Background :
Op18 (for oncoprotein 18, also designated stathmin, prosolin or metablastin) is a conserved, tubulin-associated, intracellular phosphoprotein. Many different phosphorylated forms of Op18 are observed, and it is expressed as two different isoforms. Op18 is considered a critical regulator of microtubulin dynamics and is downregulated by p53. It serves as a transducing protein, via phosphorylation, for a variety of cell signaling pathways and is involved in both mitosis and differentiation. Op18 is present in many cancers, including breast carcinomas, and is highly expressed in acute leukemias of different subtypes. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Op18 (A18) polyclonal antibody detects endogenous levels of Op18 protein. Immunogen :
Synthetic peptide, corresponding to the N-terminus of Human Op18. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of Op18 (A18) pAb at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:K562 whole cell lysate(10ug) Lane3:The Brain tissue lysate of Rat(10ug) Lane4:The Brain tissue lysate of Mouse(40ug) -
Immunohistochemistry (IHC) analyzes of Op18 (A18) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.