NGF (L82) polyclonal antibody 
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MSDS
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Product Name :
NGF (L82) polyclonal antibody Background :
The prototype neurotrophin is nerve growth factor (NGF), originally discovered in the 1950s as a soluble peptide promoting the survival of, and neurite outgrowth from, sympathetic ganglia. Three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) (also designated NT-5). These various neurotrophins stimulate the in vitro survival of distinct, but partially overlapping, populations of neurons. The cell surface receptors through which neurotrophins mediate their activity have been identified. For instance, the Trk A receptor is the preferential receptor for NGF, but also binds NT-3 and NT-4. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NGF (L82) polyclonal antibody detects endogenous levels of Pro-NGF protein, this antibody does not cross-react with Mature-NGF. Immunogen :
Synthetic peptide, corresponding to amino acids 51-100 of Human NGF. Conjugate :
Unconjugated Modification :
Unmodification
NGF (L82) polyclonal antibody Background :
The prototype neurotrophin is nerve growth factor (NGF), originally discovered in the 1950s as a soluble peptide promoting the survival of, and neurite outgrowth from, sympathetic ganglia. Three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) (also designated NT-5). These various neurotrophins stimulate the in vitro survival of distinct, but partially overlapping, populations of neurons. The cell surface receptors through which neurotrophins mediate their activity have been identified. For instance, the Trk A receptor is the preferential receptor for NGF, but also binds NT-3 and NT-4. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NGF (L82) polyclonal antibody detects endogenous levels of Pro-NGF protein, this antibody does not cross-react with Mature-NGF. Immunogen :
Synthetic peptide, corresponding to amino acids 51-100 of Human NGF. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of NGF (L82) pAb at 1:500 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:Panc1 whole cell lysate(40ug) Lane3:CT26 whole cell lysate(40ug) Lane4:H9C2 whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of NGF (L82) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Low-Level Laser Therapy Rescues Dendrite Atrophy via Upregulating BDNF Expression: Implications for Alzheimer's Disease
PMCID: Pubmed No.:23946409
Low-level laser therapy promotes dendrite growth via upregulating brain-derived neurotrophic factor expression
PMCID: Pubmed No.:23946409
The Role of Nerve Growth Factor in Ginsenoside Rg1-Induced Regeneration of Injured Rat Sciatic Nerve
PMCID: Pubmed No.:26529404
Low-level laser therapy promotes dendrite growth via upregulating brain-derived neurotrophic factor expression
PMCID: Pubmed No.:23946409
The Role of Nerve Growth Factor in Ginsenoside Rg1-Induced Regeneration of Injured Rat Sciatic Nerve
PMCID: Pubmed No.:26529404
Effect of nerve growth factor on the proliferation in newborn bovine testicular Sertoli cells
PMCID: Pubmed No.:32567558
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.