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EGF (R709) polyclonal antibody BS3549
  • Immunohistochemistry (IHC) analyzes of EGF (R709) pAb in paraffin-embedded human brain tissue.
Product NameEGF (R709) polyclonal antibody
Catalog No.BS3549
Swiss-ProtP01133
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsIHC
Application_allIHC: 1:50~1:200
BiowMW~ 10, 130(pro) kDa
Alternative NamePro-epidermal growth factor; Epidermal growth factor; Urogastrone; EGF
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
EGF (R709) polyclonal antibody
Background :
Epidermal growth factor (EGF) is an acid- and heat-stable 53 amino acid protein of 6 kDa originally found in rodents and humans. EGF has been shown to be a potent mitogen for a variety of cell types both in vivo and in vitro. It binds to the 170 kDa EGF receptor on the surface of cells and mediates intrinsic phos phoryl ation of the receptor on tyrosine residues. EGF has been detected in nearly all body fluids such as urine (urogastrone), saliva, milk and platelet-rich plasma.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
EGF (R709) polyclonal antibody detects endogenous levels of EGF protein.
Immunogen :
Synthetic peptide, corresponding to amino acids 681-730 of Human EGF.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Immunohistochemistry (IHC) analyzes of EGF (R709) pAb in paraffin-embedded human brain tissue.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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