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MyD88 (V220) polyclonal antibody BS3521
  • Western blot (WB) analysis of MyD88 (V220) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate
  • Immunohistochemistry (IHC) analyzes of MyD88 (V220) pAb in paraffin-embedded human brain tissue.
Product NameMyD88 (V220) polyclonal antibody
Catalog No.BS3521
Swiss-ProtQ99836
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200
BiowMW~ 35 kDa
Alternative NameMyeloid differentiation primary response protein MyD88
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
MyD88 (V220) polyclonal antibody
Background :
The myeloid differentiation protein MyD88 was originally characterized as a protein upregulated in myeloleukemic cells following IL-6-induced growth arrest and terminal differentiation. MyD88 is now known to function as an adaptor protein for the association of IRAK with the IL-1 receptor. MyD88 is functionally homologous to the adaptor protein tube in the Toll signaling pathway of Drosophilia, and both proteins are members of the Toll/IL-1R superfamily. MyD88 contains a characteristic N-terminal death domain that is essential for NFκB activation and an adjacent Toll/IL-1R homology domain (TIR domain). Collectively, these domains enable the protein-protein interactions of MyD88 with IRAK and the IL-1 receptor complex.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
MyD88 (V220) polyclonal antibody detects endogenous levels of MyD88 protein.
Immunogen :
Synthetic peptide, corresponding to amino acids 186-236 of Human MyD88.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of MyD88 (V220) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate
  • Immunohistochemistry (IHC) analyzes of MyD88 (V220) pAb in paraffin-embedded human brain tissue.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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