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ATP5J2 (G42) polyclonal antibody BS3496
  • Immunohistochemistry (IHC) analyzes of ATP5J2 (G42) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.
Product NameATP5J2 (G42) polyclonal antibody
Catalog No.BS3496
Swiss-ProtP56134
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsIHC
Application_allIHC: 1:50~1:200
BiowMW~ 11 kDa
Alternative NameATP synthase subunit f, mitochondrial; ATP5J2; ATP5JL
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
ATP5J2 (G42) polyclonal antibody
Background :
ATP5J2 is part of the complex F0 domain of the mitochondrial ATP synthase. This produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of two complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. ATP5J2 encodes the f subunit of the Fo complex. There are 2 isoforms produced by alternative splicing.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
ATP5J2 (G42) polyclonal antibody detects endogenous levels of ATP5J2 protein.
Immunogen :
Synthetic peptide, corresponding to amino acids 20-80 of Human ATP5J2.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Immunohistochemistry (IHC) analyzes of ATP5J2 (G42) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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