IFN-γ (Q69) polyclonal antibody 
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Product Name :
IFN-γ (Q69) polyclonal antibody Background :
Interferon (IFN)-γ is an antiviral and antiparasitic agent produced by CD4+/CD8+ lymphocytes and natural killer cells that undergo activation by antigens, mitogens or alloantigens. IFN-γ production modulates T cell growth and differentiation and inhibits the growth of B cells. Synthesis of IFN-γ is inducible by IL-2, FGF and EGF. The active form of IFN-γ is a homodimer with each subunit containing six helices. The dimeric structure of human IFN-γ is stabilized by non-covalent interactions through the interface of the helices. IFN-γ tranlsated precursor is 166 amino acids, including the 23 amino acid secretory sequence. Multiple forms exist due to variable glycosylation and under non-denaturing conditions due to dimers and tetramers. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
IFN-γ (Q69) polyclonal antibody detects endogenous levels of IFN-γ protein. Immunogen :
Synthetic peptide, corresponding to amino acids 31-80 of Human IFN-γ. Conjugate :
Unconjugated Modification :
Unmodification
IFN-γ (Q69) polyclonal antibody Background :
Interferon (IFN)-γ is an antiviral and antiparasitic agent produced by CD4+/CD8+ lymphocytes and natural killer cells that undergo activation by antigens, mitogens or alloantigens. IFN-γ production modulates T cell growth and differentiation and inhibits the growth of B cells. Synthesis of IFN-γ is inducible by IL-2, FGF and EGF. The active form of IFN-γ is a homodimer with each subunit containing six helices. The dimeric structure of human IFN-γ is stabilized by non-covalent interactions through the interface of the helices. IFN-γ tranlsated precursor is 166 amino acids, including the 23 amino acid secretory sequence. Multiple forms exist due to variable glycosylation and under non-denaturing conditions due to dimers and tetramers. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
IFN-γ (Q69) polyclonal antibody detects endogenous levels of IFN-γ protein. Immunogen :
Synthetic peptide, corresponding to amino acids 31-80 of Human IFN-γ. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of IFN-γ (Q69) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate -
Immunohistochemistry (IHC) analyzes of IFN-γ (Q69) pAb in paraffin-embedded human lymph node tissue. -
Immunohistochemistry (IHC) analyzes of IFN-γ (Q69) pAb in paraffin-embedded human lymph node tissue.
Interferon-Gamma Enhances TLR3 Expression and Anti-Viral Activity in Keratinocytes
PMCID: Pubmed No.:25822580
EBV-Encoded miR-BART20-5p and miR-BART8 Inhibit the IFN-γ–STAT1 Pathway Associated with Disease Progression in Nasal NK-Cell Lymphoma
PMCID: Pubmed No.:24655378
Respiratory syncytial virus infection upregulates NLRC5 and MHC I expression through RIG-I induction in airway epithelial cells.
PMCID: Pubmed No.:25972545
Arctigenin exerts anti-colitis efficacy through inhibiting the differentiation of Th1 and Th17 cells via an mTORC1-dependent pathway
PMCID: Pubmed No.:26074264
EBV-Encoded miR-BART20-5p and miR-BART8 Inhibit the IFN-γ–STAT1 Pathway Associated with Disease Progression in Nasal NK-Cell Lymphoma
PMCID: Pubmed No.:24655378
Arctigenin exerts anti-colitis efficacy through inhibiting the differentiation of Th1 and Th17 cells via an mTORC1-dependent pathway
PMCID: Pubmed No.:26074264
Respiratory Syncytial Virus Infection Upregulates NLRC5 and Major Histocompatibility Complex Class I Expression through RIG-I Induction in Airway Epithelial Cells
PMCID: Pubmed No.:25972545
Clostridium butyricum B1 alleviates high-fat diet-induced steatohepatitis in mice via enterohepatic immunoregulation
PMCID: Pubmed No.:28109017
Total fecal microbiota transplantation alleviates high-fat diet-induced steatohepatitis in mice via beneficial regulation of gut microbiota
PMCID: Pubmed No.:28484247
Resokaempferol-mediated anti-inflammatory effects on activated macrophages via the inhibition of JAK2/STAT3, NF-κB and JNK/p38 MAPK signaling pathways
PMCID: Pubmed No.:27261558
Composite probiotics alleviate type 2 diabetes by regulating intestinal microbiota and inducing GLP-1 secretion in db/db mice
PMCID: Pubmed No.:32035395
Role of Hepatic Deposited Immunoglobulin G in the Pathogenesis of Liver Damage in Systemic Lupus Erythematosus
PMCID: Pubmed No.:29988500
Siponimod ameliorates experimental autoimmune neuritis
PMCID: Pubmed No.:36788526
Pacenta polypeptide injection alleviates the fibrosis and inflammation in cigarette smoke extracts-induced BEAS-2B cells by modulating MMP-9/TIMP-1 signaling
PMCID: Pubmed No.:37437075
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.