Product Name :
eIF3ε (V116) polyclonal antibody Background :
Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex containing methionyl initiator tRNA (Met-tRNAiMet), which is base paired at the initiation codon (AUG, GUG) in eligible transcripts. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Eukaryotic initiation factor 3 (eIF3) is the largest family of eIFs and consists of at least 12 unique subunits in mammals. eIFε, also known as eIF p47, binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA and associates with the complex p170-eIF3. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
eIF3ε (V116) polyclonal antibody detects endogenous levels of eIF3ε protein. Immunogen :
Synthetic peptide, corresponding to amino acids 91-140 of Human eIF3ε. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot (WB) analysis of eIF3ε (V116) pAb at 1:1000 dilution Lane1:The Testis tissue lysate of Mouse(40ug) Lane2:The Testis tissue lysate of Rat(40ug) Lane3:Hela whole cell lysate(40ug) Lane4:Panc1 whole cell lysate(40ug)
• 
  Immunohistochemistry (IHC) analyzes of eIF3ε (V116) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---