Product Name :
COP ζ1 (I48) polyclonal antibody Background :
Membrane and vesicular trafficking in the early secretory pathway are mediated by non-Clathrin COP (coat protein) I-coated vesicles. COPI-coated vesicles mediate retrograde transport from the Golgi back to the ER and intra-Golgi transport. The cytosolic precursor of the COPI coat, the heptameric coatomer complex, is composed of two subcomplexes. The first consists of the COPB, COPG, COPD and COPZ1 subunits (also known as β-COP, γ-COP, δ-COP and ζ-1 COP, respectively), which are distantly homologous to AP Clathrin adaptor subunits. The second consists of the COPA, COPP and COPE subunits (also known as α-COP, COPP and ε-COP, respectively). Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
COP ζ1 (I48) polyclonal antibody detects endogenous levels of COP ζ1 protein. Immunogen :
Synthetic peptide, corresponding to the N-terminus of Human COP ζ1. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of COP ζ1 (I48) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:HEK293T cell lysate Lane3:sp2/0 cell lysate Lane4:H9C2 cell lysate Lane5:PC12 cell lysate
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  Immunohistochemistry (IHC) analyzes of COP ζ1 (I48) pAb in paraffin-embedded human esophageal carcinoma tissue at 1:50.showing Nucleus speckle and Cytoplasm staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
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  Immunohistochemistry (IHC) analyzes of COP ζ1 (I48) pAb in paraffin-embedded human esophageal carcinoma tissue at 1:50.showing Nucleus speckle and Cytoplasm staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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