p47-phox (P366) polyclonal antibody 
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Product Name :
p47-phox (P366) polyclonal antibody Background :
The heredity disease chronic granulomatous disease (CGF) has been linked to mutations in p47-phox and p67-phox. The cytosolic proteins p47-phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47-phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 doamin of p67-phox has been shown to interact with the proline rich domain of p47-phox, suggesting that p47-phox may faciliate the transport of p67-phox to the membrane. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p47-phox (P366) polyclonal antibody detects endogenous levels of p47-phox protein. Immunogen :
Synthetic peptide, corresponding to amino acids 330-380 of Human p47-phox. Conjugate :
Unconjugated Modification :
Unmodification
p47-phox (P366) polyclonal antibody Background :
The heredity disease chronic granulomatous disease (CGF) has been linked to mutations in p47-phox and p67-phox. The cytosolic proteins p47-phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47-phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 doamin of p67-phox has been shown to interact with the proline rich domain of p47-phox, suggesting that p47-phox may faciliate the transport of p67-phox to the membrane. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p47-phox (P366) polyclonal antibody detects endogenous levels of p47-phox protein. Immunogen :
Synthetic peptide, corresponding to amino acids 330-380 of Human p47-phox. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of p47-phox (P366) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug) Lane4:AML-12 whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of p47-phox (P366) pAb in paraffin-embedded human testis tissue.
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Clearance of Propionibacterium acnes by kupffer cells is regulated by osteopontin through modulating the expression of p47phox
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Protective effects of ginsenoside Rg1 on chronic restraint stress induced learning and memory impairments in male mice
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Diosgenin inhibits superoxide generation in FMLP-activated mouse neutrophils via multiple pathways
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NADPH oxidase 2-Dependent Oxidative Stress, Mitochondrial Damage and Apoptosis in the Ventral Cochlear Nucleus of D-galactose-Induced Aging Rats
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Arctigenin suppresses transforming growth factor-β1-induced expression of monocyte chemoattractant protein-1 and the subsequent epithelial-mesenchymal transition through reactive oxygen species-dependent ERK/NF-κB signaling pathway in renal tubular epithelial cells.
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Chinese medicine Tongxinluo reduces atherosclerotic lesion by attenuating oxidative stress and inflammation in microvascular endothelial cells.
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A bioinformatic and mechanistic study elicits the antifibrotic effect of ursolic acid through the attenuation of oxidative stress with the involvement of ERK, PI3K/Akt, and p38 MAPK signaling pathways in human hepatic stellate cells and rat liver.
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Sinomenine Attenuates Angiotensin II-Induced Autophagy via Inhibition of P47-Phox Translocation to the Membrane and Influences Reactive Oxygen Species Generation in Podocytes.
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Diosgenin inhibits superoxide generation in FMLP-activated mouse neutrophils via multiple pathways
PMCID: Pubmed No.:25246240
NADPH oxidase 2-Dependent Oxidative Stress, Mitochondrial Damage and Apoptosis in the Ventral Cochlear Nucleus of D-galactose-Induced Aging Rats
PMCID: Pubmed No.:25499316
Biochanin A protects dopaminergic neurons against lipopolysaccharide-induced damage and oxidative stress in a rat model of Parkinson's disease
PMCID: Pubmed No.:26394281
Effects of Placental Ischemia Are Attenuated by 1, 25-Dihydroxyvitamin D Treatment and Associated with Reduced Apoptosis and Increased Autophagy
PMCID: Pubmed No.:26562100
Elevation of cortical C26: 0 due to the decline of peroxisomal β-oxidation potentiates amyloid β generation and spatial memory deficits via oxidative stress
PMCID: Pubmed No.:26687434
Sinomenine Attenuates Angiotensin II-Induced Autophagy via Inhibition of P47-Phox Translocation to the Membrane and Influences Reactive Oxygen Species Generation in Podocytes.
PMCID: Pubmed No.:26919366
Protective effects of ginsenoside Rg1 on chronic restraint stress induced learning and memory impairments in male mice
PMCID: Pubmed No.:24560910
Elevation of cortical C26:0 due to the decline of peroxisomal β-oxidation potentiates amyloid β generation and spatial memory deficits via oxidative stress in diabetic rats
PMCID: Pubmed No.:26687434
High glucose induced endothelial cell reactive oxygen species via OGG1/PKC/NADPH oxidase pathway
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.