Product Name :
HINT1 (N99) polyclonal antibody Background :
Hint1, or histidine triad nucleotide-binding protein 1, belongs to the first branch of the HIT superfamily. It hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2. Hint1 also inhibits the activity of the transcription factors AP1, MITF and USF2, as well as influences the transcription process of the Wnt/beta-catenin pathway. In addition, mammalian Hint1 has been suggested to have some tumor suppressor activity. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Hint1 (N99) polyclonal antibody detects endogenous levels of Hint1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 80-120 of Human Hint1. Conjugate :
Unconjugated Modification :
Unmodification

• 
• 
  Immunohistochemistry (IHC) analyzes of Hint1 (N99) pAb in paraffin-embedded human prostate cancer tissue.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---