Product Name :
Laminin β-2 (C91) polyclonal antibody Background :
The Laminins comprise a growing family of disulfide-linked heterotrimers consisting of three genetically distinct polypeptide chains, designated α, β and γ. A major component of the basal lamina, Laminins play a crucial role in providing a scaffolding upon which tissues are assembled and which serves as a physical barrier separating specialized tissues. During embryogenesis and early development, cells migrate along basement membranes, which are required for the polarization of cells. The β-2 laminin chain plays a role in melanoma spread, promoting tumor migration along the abluminal surface of vessel, a phenomenon which has been termed extra-vascular migratory metastasis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Laminin β-2 (C91) polyclonal antibody detects endogenous levels of Laminin β-2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 80-130 of Human Laminin β-2. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of Laminin β-2 (C91) polyclonal antibody at 1:500 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of Laminin β-2 (C91) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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