Product Name :
SRp75 (D134) polyclonal antibody Background :
Serine/arginine-rich protein 75 (SRp75) is encoded by the SRSF4 gene. SRp75 belongs to the arginine/serine-rich factor family and likely plays a role in alternative mRNA splicing.The family of SR factors all contain one or more RNA recognition motifs (RRM) and an arginine/ serine (RS)-rich domain. They are not only essential for constitutive splicing but also regulate splicing in a concentration-dependent manner by influencing the selection of alternative splice sites. The majority of SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2/ASF, SRp20, and 9G8 are continuously shuttled between the nucleus and the cytoplasm and contribute to mRNA transport. The activity of SR proteins in regulated splicing is antagonized by members of the hnRNP A/B family of proteins, which induce drastic shifts in the selection of splicing sites. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
SRp75 (D134) polyclonal antibody detects endogenous levels of SRp75 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 100-150 of Human SRp75. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot (WB) analysis of SRp75 pAb at 1:500 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:H1792 whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug) Lane4:AML-12 whole cell lysate(40ug)
• 
  Immunohistochemistry (IHC) analyzes of SRp75 (D134) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---