HSP70 (Y41) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
HSP70 (Y41) polyclonal antibody Background :
The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. These proteins serve a variety of roles: they act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus and aid in the proper folding of nascent polypeptide chains. All members of the family, except HSP 70, are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells. Both HSP 70 and HSC 70 are involved in the chaperoning of nascent polypeptide chains and in protecting cells against the accumulation of improperly folded proteins. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
HSP70 (Y41) polyclonal antibody detects endogenous levels of HSP 70 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 20-70 of Human HSP 70. Conjugate :
Unconjugated Modification :
Unmodification
HSP70 (Y41) polyclonal antibody Background :
The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. These proteins serve a variety of roles: they act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus and aid in the proper folding of nascent polypeptide chains. All members of the family, except HSP 70, are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells. Both HSP 70 and HSC 70 are involved in the chaperoning of nascent polypeptide chains and in protecting cells against the accumulation of improperly folded proteins. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
HSP70 (Y41) polyclonal antibody detects endogenous levels of HSP 70 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 20-70 of Human HSP 70. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of HSP70 (Y41) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:NIH-3T3 whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug) Lane4:HEK293T whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of HSP70 (Y41) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.
Ischemic postconditioning prevents renal ischemia reperfusion injury through the induction of heat shock proteins in rats. (Application: Rat IHC)
PMCID: Pubmed No.:25322861
Acupuncture regulates the unfolded protein response and inhibits apoptosis in a rat model of heroin relapse
PMCID: Pubmed No.:26907924
Ischemic postconditioning prevents renal ischemia reperfusion injury through the induction of heat shock proteins in rats. (Application: Rat IHC)
PMCID: Pubmed No.:25322861
Acupuncture regulates the unfolded protein response and inhibits apoptosis in a rat model of heroin relapse
PMCID: Pubmed No.:26907924
Proteomic Analysis of Protein Expression Throughout Disease Progression in a Mouse Model of Alzheimer’s Disease
PMCID: Pubmed No.:26401771
Heat Stress Reduces Sperm Motility via Activation of Glycogen Synthase Kinase-3α and Inhibition of Mitochondrial Protein Import
PMCID: Pubmed No.:29018353
Ischemic postconditioning prevents renal ischemia reperfusion injury through the induction of heat shock proteins in rats
PMCID: Pubmed No.:25322861
iTRAQ-based quantitative proteomics reveals the neuroprotection of rhubarb in experimental intracerebral hemorrhage
PMCID: Pubmed No.:30502478
Extracellular ATPs produced in seminal plasma exosomes regulate boar sperm motility and mitochondrial metabolism
PMCID: Pubmed No.:31401476
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.