CB1 (F189) polyclonal antibody 
Datasheet
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MSDS
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Product Name :
CB1 (F189) polyclonal antibody Background :
The cannabinoid receptors (CB1 and CB2) are G-protein coupled receptors that inhibit adenylate cyclase activity in response to psychoactive cannabinoids. CB1 is expressed in brain tissue and, in low levels, in testis. Phosporylation of CB1 on serine 316 leads to a disruption of CB1-mediated inhibition of calcium channels and activation of potassium currents. This response system is thought to be involved in specific brain functions, such as nociception, control of movement, memory, and neuroendocrine regulation as well as having a possible role in brain development. In addition, CB1 may mediate the addictive behavior involved with the use of psychoactive cannabinoids, such as THC in marijuana. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CB1 (F189) polyclonal antibody detects endogenous levels of CB1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 150-200 of Human CB1. Conjugate :
Unconjugated Modification :
Unmodification
CB1 (F189) polyclonal antibody Background :
The cannabinoid receptors (CB1 and CB2) are G-protein coupled receptors that inhibit adenylate cyclase activity in response to psychoactive cannabinoids. CB1 is expressed in brain tissue and, in low levels, in testis. Phosporylation of CB1 on serine 316 leads to a disruption of CB1-mediated inhibition of calcium channels and activation of potassium currents. This response system is thought to be involved in specific brain functions, such as nociception, control of movement, memory, and neuroendocrine regulation as well as having a possible role in brain development. In addition, CB1 may mediate the addictive behavior involved with the use of psychoactive cannabinoids, such as THC in marijuana. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CB1 (F189) polyclonal antibody detects endogenous levels of CB1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 150-200 of Human CB1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of CB1 (F189) pAb at 1:500 dilution Lane1:BV2 whole cell lysate(40ug) Lane2:The Brain tissue lysate of Rat(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:U-87MG whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of CB1 (F189) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.
Cannabinoid receptor 1 disturbance of PPARγ2 augments hyperglycemia induction of mesangial inflammation and fibrosis in renal glomeruli
PMCID: Pubmed No.:24722948
Induction of Proteinuria by Cannabinoid Receptors 1 Signaling Activation in CB1 Transgenic Mice.
PMCID: Pubmed No.:25474224
Induction of Proteinuria by Cannabinoid Receptors 1 Signaling Activation in CB1 Transgenic Mice.
PMCID: Pubmed No.:25474224
Cannabinoid receptor 1 disturbance of PPARγ2 augments hyperglycemia induction of mesangial inflammation and fibrosis in renal glomeruli
PMCID: Pubmed No.:24722948
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.