CRF-RII (H112) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
CRF-RII (H112) polyclonal antibody Background :
Two CRF receptors, designated CRF-RI and CFR-RII, exhibit distinct brain localizations.Two forms of CFR-RII, designated CFR-RIIα and CFR-RIIβ, result from alternative mRNA splicing. Urocortin, an additional member of the CRF family, shares 63% sequence identity with urotensin and 45% sequence identity with CRF. Urocortin specifically binds to and activates CRF-RI and CRF-RII, but binds to CRF-RII more efficiently than CRF, suggesting that it may be the true, high affinity ligand for the CRF receptor type II. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CRF-RII (H112) polyclonal antibody detects endogenous levels of CRF-RII protein. Immunogen :
Synthetic peptide, corresponding to amino acids 96-85 of Human CRF-RII. Conjugate :
Unconjugated Modification :
Unmodification
CRF-RII (H112) polyclonal antibody Background :
Two CRF receptors, designated CRF-RI and CFR-RII, exhibit distinct brain localizations.Two forms of CFR-RII, designated CFR-RIIα and CFR-RIIβ, result from alternative mRNA splicing. Urocortin, an additional member of the CRF family, shares 63% sequence identity with urotensin and 45% sequence identity with CRF. Urocortin specifically binds to and activates CRF-RI and CRF-RII, but binds to CRF-RII more efficiently than CRF, suggesting that it may be the true, high affinity ligand for the CRF receptor type II. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CRF-RII (H112) polyclonal antibody detects endogenous levels of CRF-RII protein. Immunogen :
Synthetic peptide, corresponding to amino acids 96-85 of Human CRF-RII. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of CRF-RII (H112) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:sp2/0 cell lysate Lane3:H9C2 cell lysate
CRH suppressed TGFβ1-induced Epithelial-Mesenchymal Transition via induction of E-cadherin in breast cancer cells
PMCID: Pubmed No.:24412750
Corticotropin-releasing hormone receptors mediate apoptosis via cytosolic calcium-dependent phospholipase A2 and migration in prostate cancer cell RM-1
PMCID: Pubmed No.:24776847
Urocortin 2 but not urocortin 3 promotes the synaptic formation in hipppocampal neurons via induction of NGF production by astrocytes
PMCID: Pubmed No.:26713785
Urocortin 2 but not urocortin 3 promotes the synaptic formation in hipppocampal neurons via induction of NGF production by astrocytes
PMCID: Pubmed No.:26713785
Corticotropin-releasing hormone receptors mediate apoptosis via cytosolic calcium-dependent phospholipase A2 and migration in prostate cancer cell RM-1
PMCID: Pubmed No.:24776847
CRH suppressed TGFβ1-induced Epithelial-Mesenchymal Transition via induction of E-cadherin in breast cancer cells
PMCID: Pubmed No.:24412750
Effects of chronic noise on the corticotropin-releasing factor system in the rat hippocampus: relevance to Alzheimer’s disease-like tau hyperphosphorylation
PMCID: Pubmed No.:29228900
Effects of chronic noise on mRNA and protein expression of CRF family molecules and its relationship with p-tau in the rat prefrontal cortex
PMCID: Pubmed No.:27538655
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.