CRF-RI (H177) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
CRF-RI (H177) polyclonal antibody Background :
A high affinity CRF binding protein, designated CRF-BP, has been discovered in postmortem brain samples from AD patients. CRF-BP serves to bind and inactivate CRF, reducing the pool of “free CRF” available to bind CRFRs. Two CRF receptors, designated CRF-RI and CFR-RII, exhibit distinct brain localizations.Two forms of CFR-RII, designated CFR-RIIα and CFR-RIIβ, result from alternative mRNA splicing. Urocortin, an additional member of the CRF family, shares 63% sequence identity with urotensin and 45% sequence identity with CRF. Urocortin specifically binds to and activates CRF-RI and CRF-RII, but binds to CRF-RII more efficiently than CRF, suggesting that it may be the true, high affinity ligand for the CRF receptor type II. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CRF-RI (H177) polyclonal antibody detects endogenous levels of CRF-RI protein. Immunogen :
Synthetic peptide, corresponding to amino acids 150-200 of Human CRF-RI. Conjugate :
Unconjugated Modification :
Unmodification
CRF-RI (H177) polyclonal antibody Background :
A high affinity CRF binding protein, designated CRF-BP, has been discovered in postmortem brain samples from AD patients. CRF-BP serves to bind and inactivate CRF, reducing the pool of “free CRF” available to bind CRFRs. Two CRF receptors, designated CRF-RI and CFR-RII, exhibit distinct brain localizations.Two forms of CFR-RII, designated CFR-RIIα and CFR-RIIβ, result from alternative mRNA splicing. Urocortin, an additional member of the CRF family, shares 63% sequence identity with urotensin and 45% sequence identity with CRF. Urocortin specifically binds to and activates CRF-RI and CRF-RII, but binds to CRF-RII more efficiently than CRF, suggesting that it may be the true, high affinity ligand for the CRF receptor type II. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CRF-RI (H177) polyclonal antibody detects endogenous levels of CRF-RI protein. Immunogen :
Synthetic peptide, corresponding to amino acids 150-200 of Human CRF-RI. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of CRF-RI (H177) pAb at 1:1000 dilution Lane1:L02 whole cell lysate(20ug) Lane2:MCF-7 whole cell lysate(20ug) Lane3:A549 whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug) Lane5:BV2 whole cell lysate(40ug)
Sex difference in urocortin production is contributory to the gender disparity in a rat model of vasculitis induced by sodium laurate.
PMCID: Pubmed No.:24064363
CRH suppressed TGFβ1-induced Epithelial-Mesenchymal Transition via induction of E-cadherin in breast cancer cells
PMCID: Pubmed No.:24412750
Corticotropin-releasing hormone receptors mediate apoptosis via cytosolic calcium-dependent phospholipase A2 and migration in prostate cancer cell RM-1
PMCID: Pubmed No.:24776847
Aging as a Precipitating Factor in Chronic Restraint Stress-Induced Tau Aggregation Pathology, and the Protective Effects of Rosmarinic Acid
PMCID: Pubmed No.:26577520
Sex difference in urocortin production is contributory to the gender disparity in a rat model of vasculitis induced by sodium laurate.
PMCID: Pubmed No.:24064363
Aging as a Precipitating Factor in Chronic Restraint Stress-Induced Tau Aggregation Pathology, and the Protective Effects of Rosmarinic Acid
PMCID: Pubmed No.:26577520
Corticotropin-releasing hormone receptors mediate apoptosis via cytosolic calcium-dependent phospholipase A2 and migration in prostate cancer cell RM-1
PMCID: Pubmed No.:24776847
CRH suppressed TGFβ1-induced Epithelial-Mesenchymal Transition via induction of E-cadherin in breast cancer cells
PMCID: Pubmed No.:24412750
Effects of chronic noise on the corticotropin-releasing factor system in the rat hippocampus: relevance to Alzheimer’s disease-like tau hyperphosphorylation
PMCID: Pubmed No.:29228900
Aging as a Precipitating Factor in Chronic Restraint Stress-Induced Tau Aggregation Pathology, and the Protective Effects of Rosmarinic Acid
PMCID: Pubmed No.:26577520
Effects of chronic noise on mRNA and protein expression of CRF family molecules and its relationship with p-tau in the rat prefrontal cortex
PMCID: Pubmed No.:27538655
CRH/CRHR1 mediates prenatal synthetic glucocorticoid programming of depression-like behavior across 2 generations
PMCID: Pubmed No.:29543532
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.