MYH (Y176) polyclonal antibody 
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COA
MSDS
SHIP
Product Name :
MYH (Y176) polyclonal antibody Background :
MYH (mutY homolog (E. coli )) is a DNA glycosylase mismatch repair enzyme that in conjunction with mutM (OGG1), cleaves adenine residues paired with either oxidized (8-hydroxyguanines) or non-modified guanines in order to correct A/G and A/C mismatches. Repair of most modified and mispaired bases in the genome is initiated by DNA glycosylases, which bind and cleave N-glycosyl bonds to initiate base excision repair. MYH is crucial for the avoidance of mutations resulting from oxidative DNA damage. Multiple N-terminal splice variants of MYH exist in mammalian cells. Increasing levels of MYH in A549 cells exposed to oxygen and infrared radiation leads to improvements in cell survival. Biallelic MYH germ-line mutations predispose humans to colorectal adenomas and carcinomas. MYH is abundant in neurons where mitochondrial genomes exposed to reactive oxygen species (ROS) that damage DNA must maintain integrity over the entire mammalian life span. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
MYH (Y176) polyclonal antibody detects endogenous levels of MYH protein. Immunogen :
Synthetic peptide, corresponding to amino acids 142-190 of Human MYH. Conjugate :
Unconjugated Modification :
Unmodification
MYH (Y176) polyclonal antibody Background :
MYH (mutY homolog (E. coli )) is a DNA glycosylase mismatch repair enzyme that in conjunction with mutM (OGG1), cleaves adenine residues paired with either oxidized (8-hydroxyguanines) or non-modified guanines in order to correct A/G and A/C mismatches. Repair of most modified and mispaired bases in the genome is initiated by DNA glycosylases, which bind and cleave N-glycosyl bonds to initiate base excision repair. MYH is crucial for the avoidance of mutations resulting from oxidative DNA damage. Multiple N-terminal splice variants of MYH exist in mammalian cells. Increasing levels of MYH in A549 cells exposed to oxygen and infrared radiation leads to improvements in cell survival. Biallelic MYH germ-line mutations predispose humans to colorectal adenomas and carcinomas. MYH is abundant in neurons where mitochondrial genomes exposed to reactive oxygen species (ROS) that damage DNA must maintain integrity over the entire mammalian life span. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
MYH (Y176) polyclonal antibody detects endogenous levels of MYH protein. Immunogen :
Synthetic peptide, corresponding to amino acids 142-190 of Human MYH. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of MYH (Y176) pAb at 1:500 dilution Lane1:K562 whole cell lysate(40ug) Lane2:U-87MG whole cell lysate(40ug) Lane3:C6 whole cell lysate(40ug) Lane4:AML-12 whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of MYH (Y176) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.
Chronic exposure to microcystin-LR affected mitochondrial DNA maintenance and caused pathological changes of lung tissue in mice
PMCID: Pubmed No.:26706032
Reduced expression of MUTYH with suppressive activity against mutations caused by 8-hydroxyguanine is a novel predictor of a poor prognosis in human gastric cancer
PMCID: Pubmed No.:21826668
The Polymorphic AluYb8 Insertion in the MUTYH Gene is Associated with Reduced Type 1 Protein Expression and Reduced Mitochondrial DNA Content
PMCID: Pubmed No.:23936466
Chronic exposure to microcystin-LR affected mitochondrial DNA maintenance and caused pathological changes of lung tissue in mice
PMCID: Pubmed No.:26706032
Regulation of MUTYH, a DNA Repair Enzyme, in Renal Proximal Tubular Epithelial Cells
PMCID: Pubmed No.:26576226
Chronic exposure to microcystin-LR affected mitochondrial DNA maintenance and caused pathological changes of lung tissue in mice
PMCID: Pubmed No.:26706032
Regulation of MUTYH, a DNA Repair Enzyme, in Renal Proximal Tubular Epithelial Cells
PMCID: Pubmed No.:26576226
Regulation of MUTYH, a DNA Repair Enzyme, in Renal Proximal Tubular Epithelial Cells
PMCID: Pubmed No.:26576226
AluYb8 insertion polymorphism in the MUTYH gene impairs mitochondrial DNA maintenance and affects the age of onset of IPF
PMCID: Pubmed No.:30716719
Atorvastatin ameliorates the contractile dysfunction of the aorta induced by organ culture
PMCID: Pubmed No.:30182188
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.