Product Name :
Aiolos (S386) polyclonal antibody Background :
Ikaros family members, including Ikaros and Helios, are nuclear factors that colocalize with DNA replication machinery components in higher-order chromatin structures and respond to signaling events, such as T cell activation. Helios and Ikaros bind to similar DNA sequences and they function as hemopoietic-specific transcription factors. Members of the Ikaros family contain zinc-finger domains that are involved in DNA-binding and in the formation of homodimers and heterodimers between Ikaros family members. Aiolos, also known as zinc finger protein Ikaros 3 or ZNFN1A3, is a 509 amino acid nuclear protein. Aiolos plays an important role in lymphocyte differentiation regulation and, via this role, mutated Aiolos is implicated in leukemogenesis. Expressed in most tissues, Aiolos is predominantly found in spleen, thymus and peripheral blood leukocytes. Aiolos contains six C2H2-type zinc fingers, a motif commonly involved in nucleotide binding. Aiolos interacts with Ikaros family members, including Eos and Pegasus. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Aiolos (S386) polyclonal antibody detects endogenous levels of Aiolos (S386) protein. Immunogen :
Synthetic peptide, corresponding to Human MYLK. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of Aiolos (S386) polyclonal antibody at 1:500 dilution Lane1:BV2 whole cell lysate(40ug) Lane2:C6 whole cell lysate(40ug) Lane3:K562 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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