Product Name :
Endo180 (G145) polyclonal antibody Background :
Endo180 (Endocytic receptor 180), also known as macrophage mannose receptor C type 2 (MRC2), urokinase plasminogen activator receptor-associated protein (uPARAP) or CD280 antigen, is a ubiquitously expressed, type I membrane protein with predominant expression in fetal lung and kidney. The extracellular domain of Endo180 contains a fibronectin type II domain, eight C-type lectin domains and a cysteine-rich domain. Endo180 functions as a cell surface receptor and mediates collagen matrix remodeling as well as cell migration by playing a role in the uptake and lysosomal degradation of collagen. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Endo180 (G145) polyclonal antibody detects endogenous levels of Endo180 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 114-160 of Human Endo180. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of Endo180 (G145) pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:Panc1 whole cell lysate(40ug) Lane3:U-87MG whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of Endo180 (G145) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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