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TCF-3 (N601) polyclonal antibody BS2466
  • Immunohistochemistry (IHC) analyzes of TCF-3 (N601) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.
Product NameTCF-3 (N601) polyclonal antibody
Catalog No.BS2466
Swiss-ProtP15923
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsIHC
Application_allIHC: 1:50~1:200
BiowMW~ 60 kDa
Alternative NameTranscription factor E2-alpha; Class B basic helix-loop-helix protein 21; bHLHb21; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; Kappa-E2-binding factor; Transcription factor 3; TCF-3; Transcription factor ITF-1; TCF3; BHLHB21; E2A; ITF1
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
TCF-3 (N601) polyclonal antibody
Background :
Heterodimers between TCF3 and tissue specific basic helix loop helix (bHLH) proteins play major roles in determining tissue specific cell fate during embryogenesis, like muscle or early B cell differentiation. Dimers bind DNA on E box motifs: 5' CANNTG 3'. Binds to the kappa E2 site in the kappa immunoglobulin gene enhancer.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
TCF-3 (N601) polyclonal antibody detects endogenous levels of TCF-3 protein.
Immunogen :
Synthetic peptide, corresponding to amino acids 570-620 of Human TCF-3.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Immunohistochemistry (IHC) analyzes of TCF-3 (N601) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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