Product Name :
TWEAK (P73) polyclonal antibody Background :
TWEAK is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. It is widely expressed, has high constitutive expression in heart placenta, lung, muscle, kidney, pancreas, and HUVEC. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. TWEAK can induce apoptosis via multiple pathways of cell death in a cell type specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
TWEAK (P73) polyclonal antibody detects endogenous levels of TWEAK protein. Immunogen :
Synthetic peptide, corresponding to amino acids 51-100 of Human TWEAK. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of TWEAK (P73) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:U-87MG whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:A2780 whole cell lysate(40ug) Lane5:SP2/0 whole cell lysate(40ug) Lane6:C6 whole cell lysate(40ug) Lane7:PC12 whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of TWEAK (P73) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing secreted and cell membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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