CARKL (A53) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
CARKL (A53) polyclonal antibody Background :
CARKL, also known as SHPK (sedoheptulokinase), is a 478 amino acid protein that localizes to the cytoplasm and belongs to the FGGY family of protein kinases. Expressed at high levels in kidney, pancreas and liver and at lower levels in heart, placenta, brain and lung, CARKL functions at an optimal pH of 8.5 and catalyzes the ATP-dependent phosphorylation of sedoheptulose to yield sedoheptulose 7-phosphate, an intermediate in the pentose phosphate pathway. Once phosphorylated, sedoheptulose is unable to exit the cell via the cell membrane, resulting in the containment of sedoheptulose 7-phosphate within the cell. Defects in the gene encoding CARKL are associated with cystinosis, an autosomal recessive genetic disorder of the renal tubules that is characterized by excessive urination and low blood levels of phosphates and potassium. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CARKL pAb detects endogenous levels of CARKL protein. Immunogen :
Synthetic peptide, corresponding to amino acids 333-393 of Human AKAP 95. Conjugate :
Unconjugated Modification :
Unmodification
CARKL (A53) polyclonal antibody Background :
CARKL, also known as SHPK (sedoheptulokinase), is a 478 amino acid protein that localizes to the cytoplasm and belongs to the FGGY family of protein kinases. Expressed at high levels in kidney, pancreas and liver and at lower levels in heart, placenta, brain and lung, CARKL functions at an optimal pH of 8.5 and catalyzes the ATP-dependent phosphorylation of sedoheptulose to yield sedoheptulose 7-phosphate, an intermediate in the pentose phosphate pathway. Once phosphorylated, sedoheptulose is unable to exit the cell via the cell membrane, resulting in the containment of sedoheptulose 7-phosphate within the cell. Defects in the gene encoding CARKL are associated with cystinosis, an autosomal recessive genetic disorder of the renal tubules that is characterized by excessive urination and low blood levels of phosphates and potassium. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CARKL pAb detects endogenous levels of CARKL protein. Immunogen :
Synthetic peptide, corresponding to amino acids 333-393 of Human AKAP 95. Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot (WB) analysis of CARKL (A53) pAb at 1:500 dilution Lane1:The Kidney tissue lysate of Mouse(10ug) Lane2:The Kidney tissue lysate of Rat(10ug) Lane3:Panc1 whole cell lysate(40ug) Lane4:L02 whole cell lysate(40ug)
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.