Product Name :
EPS8L2 (K30) polyclonal antibody Background :
Eps8L2 (epidermal growth factor receptor kinase substrate 8-like protein 2), also known as EPS8R2 or PP13181, is a 715 amino acid protein that localizes to the cytoplasm and belongs to the Eps8 (epidermal growth factor receptor pathway substrate 8) family. Expressed in placenta and fibroblasts, Eps8L2 functions to stimulate the guanine exchange activity of Sos 1 (son of sevenless homolog 1), a protein that promotes the exchange of Ras-bound GDP for GTP. Additionally, Eps8L2 is thought to associate with Actin and, via this association, may play a role in membrane ruffling and remodeling of the Actin cytoskeleton. Through its ability to regulate protein activation and cytoskeleton dynamics, Eps8L2 may participate in cell growth and differentiation events within the cell. Eps8L2, a protein that is expressed as two isoforms due to alternative splicing, contains one PID (phosphotyrosine interaction) domain and one SH3 domain. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
EPS8L2 polyclonal antibody detects endogenous levels of EPS8L2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 05-60 of Human EPS8L2. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of EPS8L2 (K30) polyclonal antibody at 1:500 dilution Lane1:HCT116 whole cell lysate(10ug) Lane2:A375 whole cell lysate(20ug) Lane3:LOVO whole cell lysate(40ug) Lane4:The Embryo tissue lysate of Mouse(20ug) Lane5:PC12 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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