DMPK (R30) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
DMPK (R30) polyclonal antibody Background :
Myotonic dystrophy protein kinase (DMPK) is a ulti-domain protein kinase found in muscle that is activated in response to G protein second messengers and proteolysis. DMPK is implicated in myotonic muscular dystrophy (DM), an autosomal dominant-inherited disorder that predominately affects skeletal and cardiac muscle and causes defects in cardiac conduction. DM arises through expansion of CTG repeats in the 3’-UTR of the DMPK gene. Mutant DMPK transcripts with an extended region of CUG repeats are retained in the nucleus. These transcripts also influence the expression of the DM locus-associated homeodomain protein (DMAHP)/SIX5, to mediate in part the DM phenotype. Other substrates for DMPK include myogenin, Ltype calcium channels, and Phospholemman (PLM). Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
DMPK (R30) polyclonal antibody detects endogenous levels of DMPK protein. Immunogen :
Synthetic peptide, corresponding to the N-terminus of Human DMPK. Conjugate :
Unconjugated Modification :
Unmodification
DMPK (R30) polyclonal antibody Background :
Myotonic dystrophy protein kinase (DMPK) is a ulti-domain protein kinase found in muscle that is activated in response to G protein second messengers and proteolysis. DMPK is implicated in myotonic muscular dystrophy (DM), an autosomal dominant-inherited disorder that predominately affects skeletal and cardiac muscle and causes defects in cardiac conduction. DM arises through expansion of CTG repeats in the 3’-UTR of the DMPK gene. Mutant DMPK transcripts with an extended region of CUG repeats are retained in the nucleus. These transcripts also influence the expression of the DM locus-associated homeodomain protein (DMAHP)/SIX5, to mediate in part the DM phenotype. Other substrates for DMPK include myogenin, Ltype calcium channels, and Phospholemman (PLM). Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
DMPK (R30) polyclonal antibody detects endogenous levels of DMPK protein. Immunogen :
Synthetic peptide, corresponding to the N-terminus of Human DMPK. Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot (WB) analysis of DMPK (R30) pAb at 1:500 dilution Lane1:CT26 whole cell lysate(40ug) Lane2:The Brain tissue lysate of Rat(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:L02 whole cell lysate(40ug) Lane5:HEK293T whole cell lysate(20ug)
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.