Product Name :
PBF (R151) polyclonal antibody Background :
PBF, also known as PTTG1IP (pituitary tumor-transforming 1 interacting protein), is a 180 amino acid single-pass type I membrane protein that localizes both the cytoplasm and the nucleus and contains a coiled-coil domain. Expressed ubiquitously, PBF interacts with PTTG and is thought to facilitate the nuclear translocation of PTTG, thereby allowing the PTTG-dependent transcriptional activation of fibroblast growth factor (FGF). The gene encoding PBF maps to human chromosome 21, which houses approximately 300 genes and comprises nearly 1.5% of the human genome. Chromosome 21-associated disorders include Alzheimer’s disease, amyotrophic lateral sclerosis and, most notably, Down syndrome (also known as trisomy 21). Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PBF (R151) polyclonal antibody detects endogenous levels of PBF protein. Immunogen :
Synthetic peptide, corresponding to amino acids 130-180 of Human PBF. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of PBF (R151) pAb at 1:500 dilution Lane1:Panc1 whole cell lysate(40ug) Lane2:SGC7901 whole cell lysate(40ug) Lane3:A375 whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of PBF (R151) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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