hnRNP A1 (E85) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
hnRNP A1 (E85) polyclonal antibody Background :
HnRNP A1 belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre mRNAs in the nucleus and appear to influence pre mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. hnRNP A1 has two repeats of quasi RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re imported. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
hnRNP A1 (E85) polyclonal antibody detects endogenous levels of hnRNP A1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 51-100 of Human hnRNP A1. Conjugate :
Unconjugated Modification :
Unmodification
hnRNP A1 (E85) polyclonal antibody Background :
HnRNP A1 belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre mRNAs in the nucleus and appear to influence pre mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. hnRNP A1 has two repeats of quasi RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re imported. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
hnRNP A1 (E85) polyclonal antibody detects endogenous levels of hnRNP A1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 51-100 of Human hnRNP A1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of hnRNP A1 (E85) pAb at 1:2000 dilution Lane1:L02 whole cell lysate(10ug) Lane2:MCF-7 whole cell lysate(10ug) Lane3:HEK293T whole cell lysate(10ug) Lane4:The Lung tissue lysate of Mouse(40ug) Lane5:The Lung tissue lysate of Rat(40ug) -
Immunohistochemistry (IHC) analyzes of hnRNP A1 (E85) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Apigenin Inhibits Enterovirus-71 Infection by Disrupting Viral RNA Association with trans-Acting Factors
PMCID: Pubmed No.:25330384
Apigenin Inhibits Enterovirus-71 Infection by Disrupting Viral RNA Association with trans-Acting Factors
PMCID: Pubmed No.:25330384
Antiviral Activity of Paulownia tomentosa against Enterovirus 71 of Hand, Foot, and Mouth Disease
PMCID: Pubmed No.:25744451
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.