Product Name :
Rad50 (A708) polyclonal antibody Background :
Rad52 family members (Rad50, Rad51B/C/D, Rad52, Rad54, MRE11) mediate DNA double-strand break repair (DSBR) for DNA damage that otherwise could cause cell death, mutation or neoplastic transformation. Rad51 (RECA, BRCC5) interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage. BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of Rad51. Rad52 forms a heptameric ring that binds single-stranded DNA ends and catalyzes DNA-DNA interaction necessary for the annealing of complementary strands. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Rad50 (A708) polyclonal antibody detects endogenous levels of Rad50 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 670-722 of Human Rad50. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot (WB) analysis of Rad50 (A708) polyclonal antibody at 1:500 dilution Lane1:786-O whole cell lysate(40ug) Lane2:U-87MG whole cell lysate(40ug) Lane3:SGC-7901 whole cell lysate(40ug) Lane4:SK-OVCAR3 whole cell lysate(40ug) Lane5:The Testis tissue lysate of Mouse(40ug) Lane6:The Testis tissue lysate of Rat(40ug) Lane7:C6 whole cell lysate(40ug)
• 
  Immunohistochemistry (IHC) analyzes of Rad50 (A708) pAb in paraffin-embedded human breast cancer tissue.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---