AR-α1A (Q365) polyclonal antibody 
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Product Name :
AR-α1A (Q365) polyclonal antibody Background :
α1A-adrenergic receptors (α1A-ARs) mediate actions in the sympathetic nervous system through the binding of the catecholamines, epinephrine and norepinephrine. α1A-AR couples to Gq/11 and regulates blood pressure due to changes in vascular tone and cardiac output. Alternative splicing of the ADRA1A gene generates four isoforms with distinct C-termini, and the different expression profile of these subtypes produces distinct patterns of activation. α1A-AR transcripts are abundant in heart, brain, liver and prostate. α1A-AR transcript sizes of 6.0, 4.0, 3.0, and 2.0 kb have been detected in liver. Transcripts of 6.0, 4.0 and 3.0 kb have been detected in heart, and transcripts of 6.0 and 4.0 kb have been detected in prostate Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
AR-α1A (Q365) polyclonal antibody detects endogenous levels of AR-α1A protein. Immunogen :
Synthetic peptide, corresponding to amino acids 350-400 of Human AR-α1A. Conjugate :
Unconjugated Modification :
Unmodification
AR-α1A (Q365) polyclonal antibody Background :
α1A-adrenergic receptors (α1A-ARs) mediate actions in the sympathetic nervous system through the binding of the catecholamines, epinephrine and norepinephrine. α1A-AR couples to Gq/11 and regulates blood pressure due to changes in vascular tone and cardiac output. Alternative splicing of the ADRA1A gene generates four isoforms with distinct C-termini, and the different expression profile of these subtypes produces distinct patterns of activation. α1A-AR transcripts are abundant in heart, brain, liver and prostate. α1A-AR transcript sizes of 6.0, 4.0, 3.0, and 2.0 kb have been detected in liver. Transcripts of 6.0, 4.0 and 3.0 kb have been detected in heart, and transcripts of 6.0 and 4.0 kb have been detected in prostate Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
AR-α1A (Q365) polyclonal antibody detects endogenous levels of AR-α1A protein. Immunogen :
Synthetic peptide, corresponding to amino acids 350-400 of Human AR-α1A. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of AR-α1A (Q365) pAb at 1:500 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:PC3 whole cell lysate(40ug) Lane3:LO2 whole cell lysate(40ug) Lane4:HEK293T whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of AR-α1A (Q365) pAb in paraffin-embedded human brain tissue.
Loss of A1 Adenosine Receptor Attenuates Alpha-naphthylisothiocyanate-induced Cholestatic Liver Injury in Mice
PMCID: Pubmed No.:22956627
The dopamine D1-like receptors regulate the α1A adrenergic receptor in human renal proximal tubule cells and D1-like dopamine receptor knockout mice
PMCID: Pubmed No.:25339698
The dopamine D1-like receptors regulate the α1A adrenergic receptor in human renal proximal tubule cells and D1-like dopamine receptor knockout mice
PMCID: Pubmed No.:25339698
Dopamine D1-like receptors regulate the α1A-adrenergic receptor in human renal proximal tubule cells and D1-like dopamine receptor knockout mice
PMCID: Pubmed No.:25339698
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.