Product Name :
CYP3A4/5 (F386) polyclonal antibody Background :
Cytochrome P450 3A (CYP3A) genes encode monooxygenases—enzymes which catalyze drug metabolism and the synthesis of cholesterol, steroids and other lipids. CYP3A, the most abundant p450 enzyme in human liver, is responsible for the metabolism of more than 50% of all clinical drugs. CYP3A family members localize in organs that associate with drug disposition, including the liver, gastrointestinal tract and kidney. The CYP3A cluster maps to gene locus 7q21.3-q22.1 and consists of four genes (CYP3A4, CYP3A5, CYP3A7 andCYP3A43) and two pseudogenes (CYP3A5P1 and CYP3A5P2). CYP3A4 is abundant in the endoplasmic reticulum of liver cells and upper intestinal enterocytes. CYP3A4 expression is inducible by glucocorticoids pharmacological agents. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CYP3A4/5 (F386) polyclonal antibody detects endogenous levels of CYP3A4/5 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 351-400 of Human CYP3A4. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of CYP3A4/5 (F386) pAb at 1:500 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:The Liver tissue lysate of Rat(40ug) Lane4:The Liver tissue lysate of Mouse(40ug)
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  Immunohistochemistry (IHC) analyzes of CYP3A4/5 (F386) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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