CYP27A1 (E131) polyclonal antibody 
Datasheet
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MSDS
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Product Name :
CYP27A1 (E131) polyclonal antibody Background :
CYP27A1 participates in the degradation of cholesterol to bile acids in both the classic and acidic pathways.[1] It is the initiating enzyme in the acidic pathway to bile acids, yielding oxysterols by introducing a hydroxyl group to the carbon at the 27 position in cholesterol. In the acidic pathway, it produces 27-hydroxycholesterol from cholesterol whereas in the classic or neutral pathway, it produces 3β-hydroxy-5-cholestenoic acid.It is also involved in the metabolism of vitamin D3. While CYP27A1 is present in many different tissues, its function in these tissues is largely uncharacterized. In macrophages, 27-hydroxycholesterol generated by this enzyme may be helpful against the production of inflammatory factors associated with cardiovascular disease. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CYP27A1 (E131) polyclonal antibody detects endogenous levels of CYP27A1 protein Immunogen :
Synthetic peptide, corresponding to amino acids 100-150 of Human CYP27A1. Conjugate :
Unconjugated Modification :
Unmodification
CYP27A1 (E131) polyclonal antibody Background :
CYP27A1 participates in the degradation of cholesterol to bile acids in both the classic and acidic pathways.[1] It is the initiating enzyme in the acidic pathway to bile acids, yielding oxysterols by introducing a hydroxyl group to the carbon at the 27 position in cholesterol. In the acidic pathway, it produces 27-hydroxycholesterol from cholesterol whereas in the classic or neutral pathway, it produces 3β-hydroxy-5-cholestenoic acid.It is also involved in the metabolism of vitamin D3. While CYP27A1 is present in many different tissues, its function in these tissues is largely uncharacterized. In macrophages, 27-hydroxycholesterol generated by this enzyme may be helpful against the production of inflammatory factors associated with cardiovascular disease. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CYP27A1 (E131) polyclonal antibody detects endogenous levels of CYP27A1 protein Immunogen :
Synthetic peptide, corresponding to amino acids 100-150 of Human CYP27A1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of CYP27A1 (E131) pAb at 1:500 dilution Lane1:LO2 whole cell lysate(40ug) Lane2:HepG2 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:AML-12 whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of CYP27A1 (E131) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Cholesterol deregulation induced by chronic corticosterone (CORT) stress in pectoralis major of broiler chickens.
PMCID: Pubmed No.:25038300
In Ovo Injection of Betaine Affects Hepatic Cholesterol Metabolism through Epigenetic Gene Regulation in Newly Hatched Chicks
PMCID: Pubmed No.:25860502
In Ovo Injection of Betaine Affects Hepatic Cholesterol Metabolism through Epigenetic Gene Regulation in Newly Hatched Chicks
PMCID: Pubmed No.:25860502
Prenatal betaine exposure alleviates corticosterone-induced inhibition of CYP27A1 expression in the liver of juvenile chickens associated with its promoter DNA methylation
PMCID: Pubmed No.:28039067
Activation of epithelial proliferation induced by Eimeria acervulina infection in the duodenum may be associated with cholesterol metabolism
PMCID: Pubmed No.:27050279
Maternal betaine supplementation decreases hepatic cholesterol deposition in chicken offspring with epigenetic modulation of SREBP2 and CYP7A1 genes
PMCID: Pubmed No.:32475448
Effects of chronic dexamethasone exposure on bile acid metabolism and cecal epithelia function in goats
PMCID: Pubmed No.:29803110
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.