Cox-4 (A37) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
Cox-4 (A37) polyclonal antibody Background :
Cytochrome c oxidase (COX) functions as the terminal oxidase of the respiratory chain that uses cytochrome c as an electron donor to drive a proton gradient across the inner mitochondrial membrane. The mammalian COX apoenzyme is a heteromer consisting of three mitochondrial encoded catalytic subunits and several nuclear gene encoded structural subunits. COX contains two iron-coordination sites and two copper-coordination sites. Cytochrome c oxidase IV (COX4) is a nuclear-encoded subunit of COX that may play a role in regulating COX activity. COX4 is expressed ubiquitously in adult human tissue with the strongest levels of expression in the pancreas and moderate expression levels in heart, skeletal muscle and placenta. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Cox-4 (A37) polyclonal antibody detects endogenous levels of Cox-4 protein Immunogen :
Synthetic peptide, corresponding to the N-terminus of Human Cox-4. Conjugate :
Unconjugated Modification :
Unmodification
Cox-4 (A37) polyclonal antibody Background :
Cytochrome c oxidase (COX) functions as the terminal oxidase of the respiratory chain that uses cytochrome c as an electron donor to drive a proton gradient across the inner mitochondrial membrane. The mammalian COX apoenzyme is a heteromer consisting of three mitochondrial encoded catalytic subunits and several nuclear gene encoded structural subunits. COX contains two iron-coordination sites and two copper-coordination sites. Cytochrome c oxidase IV (COX4) is a nuclear-encoded subunit of COX that may play a role in regulating COX activity. COX4 is expressed ubiquitously in adult human tissue with the strongest levels of expression in the pancreas and moderate expression levels in heart, skeletal muscle and placenta. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Cox-4 (A37) polyclonal antibody detects endogenous levels of Cox-4 protein Immunogen :
Synthetic peptide, corresponding to the N-terminus of Human Cox-4. Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot (WB) analysis of Cox-4 (A37) polyclonal antibody at 1:500 dilution Lane1:K562 whole cell lysate(40ug) Lane2:HepG2 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:LO2 whole cell lysate(40ug) Lane5:A549 whole cell lysate(40ug) Lane6:C6 whole cell lysate(40ug) Lane7:AML-12 whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of Cox-4 (A37) pAb in paraffin-embedded human colon carcinoma tissue.
NG, a novel PABA/NO-based oleanolic acid derivative, induces human hepatoma cell apoptosis via a ROS/MAPK- dependent mitochondrial pathway
PMCID: Pubmed No.:22824464
SC-III3, a novel scopoletin derivative, induces autophagy of human hepatoma HepG2 cells through AMPK/mTOR signaling pathway by acting on mitochondria
PMCID: Pubmed No.:25964188
LZ-106, a novel analogue of enoxacin, inducing apoptosis via activation of ROS-dependent DNA damage response in NSCLCs
PMCID: Pubmed No.:27012423
LZ-106, a novel analogue of enoxacin, inducing apoptosis via activation of ROS-dependent DNA damage response in NSCLCs
PMCID: Pubmed No.:27012423
Endoplasmic Reticulum Stress Mediates Methamphetamine-Induced Blood–Brain Barrier Damage
PMCID: Pubmed No.:28959203
Sodium Butyrate Ameliorates High-Fat-Diet-Induced Non-alcoholic Fatty Liver Disease through Peroxisome Proliferator-Activated Receptor α-Mediated Activation of β Oxidation and Suppression of Inflammation
PMCID: Pubmed No.:29961332
Phytoestrogen arctigenin preserves the mucus barrier in inflammatory bowel diseases by inhibiting goblet cell apoptosis via the ERβ/TRIM21/PHB1 pathway
PMCID: Pubmed No.:35599350
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.