Product Name :
COL6A2 (Y711) polyclonal antibody Background :
Collagens (COLs) are fibrous, extracellular matrix proteins with high tensile strength that function as the major components of connective tissue, such as tendons and cartilage. All COL proteins contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Existing as a trimer with two other type VI alpha proteins, COL6A2 acts as a cell-binding protein that plays an important role in the organization of matrix components. Defects in the gene encoding COL6A2 are associated with Bethlem myopathy (BM) and Ullrich congenital muscular dystrophy (UCMD). Multiple isoforms of COL6A2 exist due to alternative splicing events. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
COL6A2 (Y711) polyclonal antibody detects endogenous levels of COL6A2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 681-730 of Human COL6A2. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of COL6A2 (Y711) pAb at 1:1000 dilution Lane1:U-87MG whole cell lysate(40ug) Lane2:PC3 whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of COL6A2 (Y711) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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