PERK (R87) polyclonal antibody 
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Product Name :
PERK (R87) polyclonal antibody Background :
An interferon-inducible, RNA-dependent protein serine /threonine kinase (PKR) has been described. PKR in earlier literature is variously known as DAI, dsJ, PI kinase, p65, p67 or TIK for the mouse kinase; and p68 or p69 for the human kinase. The PKR kinase substrate is the α subunit of protein synthesis initiation factor eIF-2. Phosphorylation of eIF-2α on serine-51 results in inhibition of translation. The serine /threonine kinase catalytic domains map to the carboxy terminal half of the protein while the RNA-binding domains are located in the amino terminal region. PERK is a type I transmembrane protein located in the endoplasmic reticulum (ER) that contains a kinase domain similar to the kinase domain of PKR. PERK is activated in response to ER stress and phosphorylates eIF-2α, thus inhibiting the translation of mRNA. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PERK (R87) polyclonal antibody detects endogenous levels of PERK protein. Immunogen :
Synthetic peptide, corresponding to amino acids 56-102 of Human PERK. Conjugate :
Unconjugated Modification :
Unmodification
PERK (R87) polyclonal antibody Background :
An interferon-inducible, RNA-dependent protein serine /threonine kinase (PKR) has been described. PKR in earlier literature is variously known as DAI, dsJ, PI kinase, p65, p67 or TIK for the mouse kinase; and p68 or p69 for the human kinase. The PKR kinase substrate is the α subunit of protein synthesis initiation factor eIF-2. Phosphorylation of eIF-2α on serine-51 results in inhibition of translation. The serine /threonine kinase catalytic domains map to the carboxy terminal half of the protein while the RNA-binding domains are located in the amino terminal region. PERK is a type I transmembrane protein located in the endoplasmic reticulum (ER) that contains a kinase domain similar to the kinase domain of PKR. PERK is activated in response to ER stress and phosphorylates eIF-2α, thus inhibiting the translation of mRNA. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PERK (R87) polyclonal antibody detects endogenous levels of PERK protein. Immunogen :
Synthetic peptide, corresponding to amino acids 56-102 of Human PERK. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of PERK (R87) pAb at 1:1000 dilution Lane1:HCT116 whole cell lysate(20ug) Lane2:MCF-7 whole cell lysate(20ug) Lane3:A375 whole cell lysate(20ug) Lane4:CT26 whole cell lysate(40ug) Lane5:PMVEC whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of PERK (R87) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase. -
Immunohistochemistry (IHC) analyzes of PERK (R87) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase. -
Immunohistochemistry (IHC) analyzes of PERK (R87) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
E platinum, a newly synthesized platinum compound, induces apoptosis through ROS‐triggered ER stress in gastric carcinoma cells
PMCID: Pubmed No.:27061377
E platinum, a newly synthesized platinum compound, induces apoptosis through ROS‐triggered ER stress in gastric carcinoma cells
PMCID: Pubmed No.:27061377
Inhibition of hepatocyte nuclear factor 1b induces hepatic steatosis through DPP4/NOX1-mediated regulation of superoxide
PMCID: Pubmed No.:28942246
TBHQ Alleviated Endoplasmic Reticulum Stress-Apoptosis and Oxidative Stress by PERK-Nrf2 Crosstalk in Methamphetamine-Induced Chronic Pulmonary Toxicity
PMCID: Pubmed No.:28303170
Melatonin, a novel selective ATF-6 inhibitor, induces human hepatoma cell apoptosis through COX-2 downregulation
PMCID: Pubmed No.:28246472
E platinum, a newly synthesized platinum compound, induces apoptosis through ROS‐triggered ER stress in gastric carcinoma cells
PMCID: Pubmed No.:27061377
Endoplasmic reticulum stress and apoptosis via PERK-eIF2α-CHOP signaling in the methamphetamine-induced chronic pulmonary injury
PMCID: Pubmed No.:28081472
Endoplasmic Reticulum Stress Promotes Sorafenib Resistance via miR-188-5p/hnRNPA2B1 mediated upregulation of PKM2 in Hepatocellular Carcinoma
PMCID: Pubmed No.:34786210
KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells
PMCID: Pubmed No.:29520102
Microcystin-LR ameliorates pulmonary fibrosis via modulating CD206 + M2-like macrophage polarization
PMCID: Pubmed No.:32075954
Melatonin Increases the Sensitivity of Hepatocellular Carcinoma to Sorafenib through the PERK-ATF4-Beclin1 Pathway
PMCID: Pubmed No.:31523192
GanDouLing combined with Penicillamine improves cerebrovascular injury via PERK/eIF2α/CHOP endoplasmic reticulum stress pathway in the mouse model of Wilson’s disease
PMCID: Pubmed No.:30181379
Role of the PERK-eIF2α-CHOP Signaling Pathway in the Effect of Needle Knife Therapy on Knee Joint Chondrocyte Apoptosis
PMCID: Pubmed No.:31316574
Rosuvastatin protects against endothelial cell apoptosis in vitro and alleviates atherosclerosis in ApoE‑/‑ mice by
suppressing endoplasmic reticulum stress
PMCID: Pubmed No.:32537013
Regulation of the sensitivity of hepatocarcinoma cells by ORMDL3, to sorafenib by autophagy
PMCID: Pubmed No.:35972600
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.