Product Name :
ENX-2 (E192) polyclonal antibody Background :
In Drosophila, the Polycomb (PcG) gene family encodes chromatin proteins that are required for the repression of homeotic loci in embryonic development. PcG proteins work in conjunction with the trithorax-group (trxG) proteins, which activate homeobox gene expression during embryonic development. ENX-1, a mammalian homolog of the Drosophila gene enhancer of zeste, is a PcG protein that is ubiquitously expressed during early embryogenesis and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, ENX-1 is restricted to specific sites, including spleen, testis and placenta. ENX-2 is another mammalian homolog of the Drosophila gene enhancer of zeste and contains one SET domain. The gene for human ENX-2 maps to chromosome 17q21. ENX-2 expression is ubiquitous in adult and fetal tissue, where it may aid in maintaining heterochromatin stability. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ENX-2 (E192) polyclonal antibody detects endogenous levels of ENX-2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 162-208 of Human ENX-2. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot (WB) analysis of ENX-2 (E192) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:A2780 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:A549 whole cell lysate(40ug) Lane5:HepG2 whole cell lysate(40ug) Lane6:H9C2 whole cell lysate(40ug) Lane7:3T3-L1 whole cell lysate(40ug)
• 
  Immunohistochemistry (IHC) analyzes of ENX-2 (E192) pAb in paraffin-embedded human brain tissue.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---