Product Name :
SMG7 (K548) polyclonal antibody Background :
In humans, NMD depends on RNA-dependent ATPase and 5' to 3' helicase UPF1, plus six other proteins designated SMG1, SMG5, SMG6, SMG7, UPF2 and UPF3. SMG5, SMG7 and UPF1 localize to cytoplasmic foci called P-bodies, while SMG5, SMG6 and SMG7 target UPF1 for dephosphorylation. SMG7 may also act as an adaptor in targeting mRNAs associated with phosphorylated UPF1 for degradation. SMG7 provides a link between the NMD pathway and mRNA degradation machinery by forming a complex with the proteins SMG5 and UPF1, interacting with them via its N-terminal domain, and targeting bound reporter transcripts for decay via its C-terminal domain. SMG7 contains a 14-3-3-like domain, and residues that bind phosphoserine-containing peptides in 14-3-3 proteins are conserved at the equivalent positions in SMG7. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
SMG7 (K548) polyclonal antibody detects endogenous levels of SMG7 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 512-564 of Human SMG7. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of SMG7 (K548) pAb at 1:500 dilution Lane1:K562 whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:U-87MG whole cell lysate(40ug) Lane4:SGC7901 whole cell lysate(40ug) Lane5:PC12 whole cell lysate(40ug) Lane6:BV2 whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of SMG7 (K548) pAb in paraffin-embedded human tonsil cancer tissue at 1:50.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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