Product Name :
C/EBP-ε (K220) polyclonal antibody Background :
C/EBP α regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP α uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic α helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP α appears to function exclusively in terminally-differentiated, growth-arrested cells. Additional family members include C/EBP β, C/EBP γ, C/EBP δ and C/EBP ε, all of which exhibit similar DNA-binding specificities and affinities to C/EBP α. Furthermore, C/EBP β and C/EBP δ readily form heterodimers both with each other as well as with C/EBP α. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
C/EBP-ε (K220) polyclonal antibody detects endogenous levels of C/EBP-ε protein. Immunogen :
Synthetic peptide, corresponding to amino acids 188-245 of Human C/EBP-ε. Conjugate :
Unconjugated Modification :
Unmodification

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  Immunohistochemistry (IHC) analyzes of C/EBP-ε (K220) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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