WTAP (H351) polyclonal antibody 
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Product Name :
WTAP (H351) polyclonal antibody Background :
Wilms’ tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and is observed in both sporadic and inherited forms. The Wilms’ tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators, and functions as a transcriptional repressor. WTAP (Wilms tumor 1-associating protein) is a ubiquitously expressed nuclear protein that interacts with WT1 and may be involved in regulating mRNA splicing. WTAP is found in nuclear speckles, where it regulates the G2/M cell cycle transition by binding to the 3’ UTR of cyclin A2, thus enhancing its stability. Additionally, WTAP inhibits expression of WT1 target genes and is able to impair the ability of WT1 to bind DNA. Two isoforms of WTAP exist due to alternative splicing events. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
WTAP (H351) polyclonal antibody detects endogenous levels of WTAP protein. Immunogen :
Synthetic peptide, corresponding to amino acids 320-374 of Human WTAP. Conjugate :
Unconjugated Modification :
Unmodification
WTAP (H351) polyclonal antibody Background :
Wilms’ tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and is observed in both sporadic and inherited forms. The Wilms’ tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators, and functions as a transcriptional repressor. WTAP (Wilms tumor 1-associating protein) is a ubiquitously expressed nuclear protein that interacts with WT1 and may be involved in regulating mRNA splicing. WTAP is found in nuclear speckles, where it regulates the G2/M cell cycle transition by binding to the 3’ UTR of cyclin A2, thus enhancing its stability. Additionally, WTAP inhibits expression of WT1 target genes and is able to impair the ability of WT1 to bind DNA. Two isoforms of WTAP exist due to alternative splicing events. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
WTAP (H351) polyclonal antibody detects endogenous levels of WTAP protein. Immunogen :
Synthetic peptide, corresponding to amino acids 320-374 of Human WTAP. Conjugate :
Unconjugated Modification :
Unmodification
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Immunohistochemistry (IHC) analyzes of WTAP (H351) pAb in paraffin-embedded human tonsil cancer tissue at 1:50.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.