LT-β (V211) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
LT-β (V211) polyclonal antibody Background :
Tumor necrosis factor (TNF) and lymphotoxin-alpha (LT-α, also known as TNF−β) are members of a family of secreted and cell surface cytokines that participate in the regulation of immune and inflammatory responses. LT-β (lymphotaxin-beta or tumor necrosis factor C) is a type II membrane protein with significant homology to TNF, LT-α, and the ligand for the CD40 receptor. LT-α is present on the surface of activated T, B, and LAK cells as a complex with the 33 kda glycoprotein, LT-β. LT-β, also expressed by active lymphocytes, forms a heterotrimer with LT-a on the cell surface and anchors LT-α to the cell surface. A TNF receptor-related protein, the LT-β receptor (also known as TNFC receptor), is the human receptor for the LT-α/LT-β heterotrimer. There are two LT-β isoforms expressed in human lymphoid cell lines and non-Hodgkin's lymphomas. The gene which encodes LT-β maps to the major histocompatibility complex region on human chromosome 6p21.3. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
LT-β (V211) polyclonal antibody detects endogenous levels of LT-β protein. Immunogen :
Synthetic peptide, corresponding to amino acids 200-250 of Human LT-β. Conjugate :
Unconjugated Modification :
Unmodification
LT-β (V211) polyclonal antibody Background :
Tumor necrosis factor (TNF) and lymphotoxin-alpha (LT-α, also known as TNF−β) are members of a family of secreted and cell surface cytokines that participate in the regulation of immune and inflammatory responses. LT-β (lymphotaxin-beta or tumor necrosis factor C) is a type II membrane protein with significant homology to TNF, LT-α, and the ligand for the CD40 receptor. LT-α is present on the surface of activated T, B, and LAK cells as a complex with the 33 kda glycoprotein, LT-β. LT-β, also expressed by active lymphocytes, forms a heterotrimer with LT-a on the cell surface and anchors LT-α to the cell surface. A TNF receptor-related protein, the LT-β receptor (also known as TNFC receptor), is the human receptor for the LT-α/LT-β heterotrimer. There are two LT-β isoforms expressed in human lymphoid cell lines and non-Hodgkin's lymphomas. The gene which encodes LT-β maps to the major histocompatibility complex region on human chromosome 6p21.3. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
LT-β (V211) polyclonal antibody detects endogenous levels of LT-β protein. Immunogen :
Synthetic peptide, corresponding to amino acids 200-250 of Human LT-β. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of LT-β (V211) pAb at 1:500 dilution Lane1:H9C2 whole cell lysate(40ug) Lane2:MEF whole cell lysate(40ug) Lane3:Hela whole cell lysate(40ug) Lane4:HEK293T whole cell lysate(40ug) Lane5:HepG2 whole cell lysate(40ug) Lane6:A549 whole cell lysate(40ug)
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.