Product Name :
UMP-CMP Kinase (E36) polyclonal antibody Background :
Nucleoside monophosphate kinases are required for pharmacological activation of therapeutic nucleosides and nucleotide analogs. CMPK (Cytidine monophosphate kinase), also known as UMP-CMP kinase and Deoxycytidylate kinase, is a 196 amino acid protein that catalyzes the phosphoryl transfer from ATP to UMP, CMP and dCMP. This enzymatic reaction leads to the formation of ADP and the corresponding nucleoside diphosphate, which are required for cellular nucleic acid synthesis. Primarily localized to the cytoplasm, CMPK also plays an important role in the activation of pyrimidine analogs, which is clinically useful anti-cancer and anti-viral drugs. CMP is the best substrate for CMPK, followed by UMP and dCMP. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
UMP-CMP Kinase (E36) polyclonal antibody detects endogenous levels of UMP-CMP Kinase protein. Immunogen :
Synthetic peptide, corresponding to the N-terminal of Human UMP-CMP Kinase. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of UMP-CMP Kinase (E36) pAb at 1:1000 dilution Lane1:Hela whole cell lysate(40ug) Lane2:H9C2 whole cell lysate(40ug) Lane3:MEF whole cell lysate(40ug) Lane4:A549 whole cell lysate(40ug) Lane5:HEK293T whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of UMP-CMP Kinase (E36) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
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  Immunohistochemistry (IHC) analyzes of UMP-CMP Kinase (E36) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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