Product Name :
TCF-4/12 (S616) polyclonal antibody Background :
T cell factors (TCFs) comprise a family of DNA-binding transcriptional activators that are essential for lymphoid cell development. These transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, which mediates DNA binding. TCF-4 mainly localizes in the cytoplasm and is transported into the nucleus directly bound to β-catenin in a cooperative manner. This TCF-4/β-catenin complex induces expression of Wnt target genes, including multiple cancer-associated genes. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
TCF-4/12 (S616) polyclonal antibody detects endogenous levels of TCF-4, and also detects TCF-12 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 580-630 of Human TCF-4. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of TCF-4/12 (S616) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate(20ug) Lane2:HepG2 whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug) Lane4:A549 whole cell lysate(40ug) Lane5:PMVEC whole cell lysate(40ug) Lane6:AML-12 whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of TCF-4/12 (S616) pAb in paraffin-embedded human brain tissue.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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