Creatine Kinase M (Y14) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
Creatine Kinase M (Y14) polyclonal antibody Background :
Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. The CKM isoform, predominant in skeletal muscle and heart tissue, is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Creatine Kinase M (Y14) polyclonal antibody detects endogenous levels of Creatine Kinase M protein. Immunogen :
Synthetic peptide, corresponding to the N-terminus of Human Creatine Kinase M. Conjugate :
Unconjugated Modification :
Unmodification
Creatine Kinase M (Y14) polyclonal antibody Background :
Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. The CKM isoform, predominant in skeletal muscle and heart tissue, is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Creatine Kinase M (Y14) polyclonal antibody detects endogenous levels of Creatine Kinase M protein. Immunogen :
Synthetic peptide, corresponding to the N-terminus of Human Creatine Kinase M. Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot (WB) analysis of Creatine Kinase M (Y14) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:NIH-3T3 cell lysate Lane3:H9C2 cell lysate -
Immunohistochemistry (IHC) analyzes of Creatine Kinase M (Y14) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.
Slow skeletal muscle myosin binding protein-C (MyBPC1) mediates recruitment of muscle type creatine kinase (CK) to myosin.
PMCID: Pubmed No.:21426302
Metallothioneins Protect Cytosolic Creatine kinases Against Stress Induced by Nitrogen-Based Oxidants
PMCID: Pubmed No.:21967612
Directed Myogenic Differentiation of Human Induced Pluripotent Stem Cells
PMCID: Pubmed No.:25971915
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.