Product Name :
TBX15/18 (D164) polyclonal antibody Background :
T-box transcription factors are a group of phylogenetically conserved genes that contain a uniquely defining DNA-binding domain, the T-box domain. These genes are believed to be involved in the regulation of development processes. In mouse embryo development, Tbx15 is expressed at the beginning of day 9.5, primarily in the craniofacial region and in the developing limbs. Twelve human T-box genes have been isolated so far. TBX 18 has been reported to be involved in numerous development processes and to act as an antiapoptotic factor. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
TBX15/18 (D164) polyclonal antibody detects endogenous levels of TBX15 and TBX18 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 131-180 of Human TBX15. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of TBX15/18 (D164) pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:The Lung tissue lysate of Rat(40ug) Lane4:AML-12 whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of TBX15/18 (D164) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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